Patient with Chronic Myelocytic Leuikemia

one-third of this protein to be in molecular weight range 20,000-40,000 (fraction BJC). BJC, prepared from 9 liters of urine by gel filtration, was chromatographed on carboxymethylcellulose. Two proteins were eluted from the resin in pure form (as shown by zone and immunoelectrophoresis) in yields representing 8 and 3 mg/liter of urine: BjC1 and BJC2. Their amino acid compositions were identical. BJC1 conitained 61% carbohydrate (33% hexose, 11% sialic acid, 13% glucosamine, 5% galactosamine). RJC2 contained onefourth to one-half as much of each carbohydrate. Molecular weight of BJC1 was estimated at 29,000 by gel filtration. Neither glycoprotein reacted with rabbit antiserum to normal human serum. Antiserum to BJC1 was made in the rabbit. Tmmunoelectrophoresis with this antiserum slhowed a faint precipitin line, corresponding in mobility to BJC1, in normal human plasma, and a stronger line in most leukemic plasmas. By immunodiffusion, BJC1 was not detectable in normal human urine, but a positive reaction occurred in the following conditions: leukemia, 64-72%; other types of disseminated neoplastic disease, 36-78%; regional ileitis, 45%; ulcerative colitis, 38%; tuberculosis, 33%; during the 1st wk after major surgery, 33%. BJC2 was found in the urine by immunoelectrophoresis in 10% of patients with neoplastic disease and was not observed in urine of other patients or in human plasma. Amino acid composition, carbohydrate content, and antigenic specificity indicate BJC1 is a previously unrecogniized member of the system of normal human Received for putblication 9 JuIAy 1973 and in revised form 22 October 197?. plasma glycoproteins. l ike certain other glycoproteins, its plasma concentrationi frequently increases in patients with neoplastic disease, chronic iniflammatory (lisease, or tuberculosis and after surgery. Because molecular weight is 29,000, increased plasma concenitration readily cauises its appearalnce in the urine.